,
Mekebeb Afework,
Eyasu Makonnen,
Wondwosen Ergete,
Asfaw Debella,
Tesfaye Tolessa,
Minale Fekadie
Introduction: Traditional medicine is an ancient medical practice that is still widely used in prevention and treatment of various health problems worldwide, including Ethiopia. M.gracilipes is one of medicinal plant in Ethiopia used for treatment of various ailments still is very common. This study evaluated the sub-chronic toxic effects of M. gracilipes aqueous leaves extract on biochemical parameters and histopathology of liver and kidneys. Methods: For sub-chronic toxicity study a total of 30 mice were used, three groups (I–III) of mice (10 animals each) were used. Group I served as control and received a vehicle while groups II and III daily administered with 700 and 2100 mg/kg extract respectively orally by using oral gavage for 90 days. At the end of the experiment, the mice were sacrificed by using diethyl ether; blood was collected for assessing biochemical parameters and histopathological evaluations on liver and kidneys were performed. Results: Sub chronic treatment of extract for 90 days, at 700 and 2100 mg/kg body weight did not induce any sign of illness and /or death and had no adverse effect on biochemical parameters and blood parameters. Liver and kidney sections also revealed normal architecture, except some pyknotic nuclei and focal mononuclear leukocytic infiltrations observed in some of the liver and kidney tissues at higher dose (2100mg/kg). Conclusion: The results of this sub chronic toxicity study showed that M. gracilipes aqueous leaves extract is safe at daily doses of 700 mg/kg body weight, even when taken for longer period. At higher doses, however, the extract may induce mild hepatorenal toxicity.
| Published in | American Journal of Bioscience and Bioengineering (Volume 12, Issue 2) |
| DOI | 10.11648/j.bio.20241202.11 |
| Page(s) | 24-31 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Aqueous Leaves Extract, Sub-Chronic Toxicity, Histopathology, Maytenus Gracilipes, Liver and Kidneys
| [1] | Robinson M, Zhang X. THE WORLD MEDICINES SITUATION 2011 TRADITIONAL MEDICINES: GLOBAL SITUATION, ISSUES AND CHALLENGES. In 2011. |
| [2] | Addis G, Abebe DT, Genebo T, Urga K. Perceptions and practices of modern and traditional health practitioners about traditional medicine in Shirka district, Arsi zone, Ethiopia. In 2002. |
| [3] | Berhanu A. Book Review: Ethiopian Traditional Medicine: Common Medicinal Plants in Perspective. J Black Stud. 2002 May 1; 32(5): 610–2. |
| [4] | De Smet PAGM. Traditional pharmacology and medicine in Africa: Ethnopharmacological themes in sub-Saharan art objects and utensils. J Ethnopharmacol. 1998 Nov 1; 63(1): 1–175. |
| [5] | Gedif T, Hahn H-J. The use of medicinal plants in self-care in rural central Ethiopia. J Ethnopharmacol. 2003 Aug 1; 87(2): 155–61. |
| [6] | Alves RR, Rosa IM. Biodiversity, traditional medicine and public health: where do they meet? J Ethnobiol Ethnomedicine. 2007 Mar 21; 3(1): 14. |
| [7] | Azaizeh H, Saad B, Khalil K, Said O. The State of the Art of Traditional Arab Herbal Medicine in the Eastern Region of the Mediterranean: A Review. Evid Based Complement Alternat Med. 2006 Jun; 3(2): 229–35. |
| [8] | Gedif T, Hahn H-J. Herbalists in Addis Ababa and Butajira, Central Ethiopia: Mode of service delivery and traditional pharmaceutical practice. Ethiop J Health Dev. 2002 Jan 1; 16(2): 183-189–189. |
| [9] | Chester AH, Yacoub MH. The role of endothelin-1 in pulmonary arterial hypertension. Glob Cardiol Sci Pract. 2014 Jun 18; 2014(2): 62–78. |
| [10] | Kumaran RS, Jung H, Kim HJ. In vitro screening of taxol, an anticancer drug produced by the fungus, Colletotrichum capsici. Eng Life Sci. 2011; 11(3): 264–71. |
| [11] | Schümann J, Prockl J, Kiemer AK, Vollmar AM, Bang R, Tiegs G. Silibinin protects mice from T cell-dependent liver injury. J Hepatol. 2003 Sep 1; 39(3): 333–40. |
| [12] | Angerhofer CK. Herbal Medicines, a Guide for Healthcare Professionals, Second Edition By Joanne Barnes (University of London), Linda A. Anderson (Medicines Control Agency, London), and J. David Phillipson (University of London). Pharmaceutical Press, London, UK. 2002. xiv + 530 pp. 18.5 × 24.5 cm. $59.95. ISBN 0-85369-474-5. J Nat Prod. 2002 Dec 1; 65(12): 1964–1964. |
| [13] | Mahomoodally MF. Traditional Medicines in Africa: An Appraisal of Ten Potent African Medicinal Plants [Internet]. Evidence-Based Complementary and Alternative Medicine. 2013 [cited 2019 Dec 27]. Available from: |
| [14] | Adebayo JO, Yakubu MT, Egwim EC, Owoyele VB, Enaibe BU. Effect of ethanolic extract of Khaya senegalensis on some biochemical parameters of rat kidney. J Ethnopharmacol. 2003 Sep 1; 88(1): 69–72. |
| [15] | Geyid A, Abebe D, Debella A, Makonnen Z, Aberra F, Teka F, et al. Screening of some medicinal plants of Ethiopia for their anti-microbial properties and chemical profiles. J Ethnopharmacol. 2005 Jan 1. |
| [16] |
Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure - en - OECD [Internet]. [cited 2019 Jul 14]. Available from:
https://www.oecd.org/env/test-no-425-acute-oral-toxicity-up-and-down-procedure-9789264071049-en.htm |
| [17] | Saleem U, Amin S, Ahmad B, Azeem H, Anwar F, Mary S. Acute oral toxicity evaluation of aqueous ethanolic extract of Saccharum munja Roxb. roots in albino mice as per OECD 425 TG. Toxicol Rep. 2017 Oct 31; 4: 580–5. |
| [18] | Rahman MF, Siddiqui MK, Jamil K. Effects of Vepacide (Azadirachta indica) on asp artate and al anine aminotransferase profiles in a subchronic study with rats. Hum Exp Toxicol. 2001 May 1; 20(5): 243–9. |
| [19] | Sonntag O. Tietz Fundamentals of Clinical Chemistry, Fifth Edition. Carl A. Burtis and Edward R. Ashwood, eds. Philadelphia: WB Saunders, 2001, 1091 pp., $75.00. ISBN 0-7216-8634-6. Clin Chem. 2002 Jan 1; 48(1): 213–213. |
| [20] | Ugwah-Oguejiofor CJ, Okoli CO, Ugwah MO, Umaru ML, Ogbulie CS, Mshelia HE, et al. Acute and sub-acute toxicity of aqueous extract of aerial parts of Caralluma dalzielii N. E. Brown in mice and rats. Heliyon [Internet]. 2019 Jan 29 [cited 2019 Jul 28]; 5(1). Available from: |
| [21] | Debelo N, Afework M. Assessment of Hematological, Biochemical and Histopathological Effects of Acute and Sub-chronic Administration of the Aqueous Leaves Extract of Thymus schimperi in Rats. J Clin Toxicol [Internet]. 2016 [cited 2019 Dec 27]; 06(02). Available from: |
| [22] | Feng Y, Cheung K-F, Wang N, Liu P, Nagamatsu T, Tong Y. Chinese medicines as a resource for liver fibrosis treatment. Chin Med. 2009 Aug 20; 4: 16. |
| [23] | Loha M, Mulu A, Abay SM, Ergete W, Geleta B. Acute and Subacute Toxicity of Methanol Extract of Syzygium guineense Leaves on the Histology of the Liver and Kidney and Biochemical Compositions of Blood in Rats [Internet]. Evidence-Based Complementary and Alternative Medicine. 2019 [cited 2019 Dec 27]. Available from: |
APA Style
Ayele, M., Afework, M., Makonnen, E., Ergete, W., Debella, A., et al. (2024). Sub Chronic Toxicity Study of Aqueous Leaves Extract of Maytenus Gracilipes on Some Biochemical Parameters and Histopathology of Liver and Kidneys in Mice. American Journal of Bioscience and Bioengineering, 12(2), 24-31. https://doi.org/10.11648/j.bio.20241202.11
ACS Style
Ayele, M.; Afework, M.; Makonnen, E.; Ergete, W.; Debella, A., et al. Sub Chronic Toxicity Study of Aqueous Leaves Extract of Maytenus Gracilipes on Some Biochemical Parameters and Histopathology of Liver and Kidneys in Mice. Am. J. BioSci. Bioeng. 2024, 12(2), 24-31. doi: 10.11648/j.bio.20241202.11
AMA Style
Ayele M, Afework M, Makonnen E, Ergete W, Debella A, et al. Sub Chronic Toxicity Study of Aqueous Leaves Extract of Maytenus Gracilipes on Some Biochemical Parameters and Histopathology of Liver and Kidneys in Mice. Am J BioSci Bioeng. 2024;12(2):24-31. doi: 10.11648/j.bio.20241202.11
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Download@article{10.11648/j.bio.20241202.11,
author = {Mengistu Ayele and Mekebeb Afework and Eyasu Makonnen and Wondwosen Ergete and Asfaw Debella and Tesfaye Tolessa and Minale Fekadie},
title = {Sub Chronic Toxicity Study of Aqueous Leaves Extract of Maytenus Gracilipes on Some Biochemical Parameters and Histopathology of Liver and Kidneys in Mice
},
journal = {American Journal of Bioscience and Bioengineering},
volume = {12},
number = {2},
pages = {24-31},
doi = {10.11648/j.bio.20241202.11},
url = {https://doi.org/10.11648/j.bio.20241202.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20241202.11},
abstract = {Introduction: Traditional medicine is an ancient medical practice that is still widely used in prevention and treatment of various health problems worldwide, including Ethiopia. M.gracilipes is one of medicinal plant in Ethiopia used for treatment of various ailments still is very common. This study evaluated the sub-chronic toxic effects of M. gracilipes aqueous leaves extract on biochemical parameters and histopathology of liver and kidneys. Methods: For sub-chronic toxicity study a total of 30 mice were used, three groups (I–III) of mice (10 animals each) were used. Group I served as control and received a vehicle while groups II and III daily administered with 700 and 2100 mg/kg extract respectively orally by using oral gavage for 90 days. At the end of the experiment, the mice were sacrificed by using diethyl ether; blood was collected for assessing biochemical parameters and histopathological evaluations on liver and kidneys were performed. Results: Sub chronic treatment of extract for 90 days, at 700 and 2100 mg/kg body weight did not induce any sign of illness and /or death and had no adverse effect on biochemical parameters and blood parameters. Liver and kidney sections also revealed normal architecture, except some pyknotic nuclei and focal mononuclear leukocytic infiltrations observed in some of the liver and kidney tissues at higher dose (2100mg/kg). Conclusion: The results of this sub chronic toxicity study showed that M. gracilipes aqueous leaves extract is safe at daily doses of 700 mg/kg body weight, even when taken for longer period. At higher doses, however, the extract may induce mild hepatorenal toxicity.
},
year = {2024}
}
TY - JOUR T1 - Sub Chronic Toxicity Study of Aqueous Leaves Extract of Maytenus Gracilipes on Some Biochemical Parameters and Histopathology of Liver and Kidneys in Mice AU - Mengistu Ayele AU - Mekebeb Afework AU - Eyasu Makonnen AU - Wondwosen Ergete AU - Asfaw Debella AU - Tesfaye Tolessa AU - Minale Fekadie Y1 - 2024/04/17 PY - 2024 N1 - https://doi.org/10.11648/j.bio.20241202.11 DO - 10.11648/j.bio.20241202.11 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 24 EP - 31 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.20241202.11 AB - Introduction: Traditional medicine is an ancient medical practice that is still widely used in prevention and treatment of various health problems worldwide, including Ethiopia. M.gracilipes is one of medicinal plant in Ethiopia used for treatment of various ailments still is very common. This study evaluated the sub-chronic toxic effects of M. gracilipes aqueous leaves extract on biochemical parameters and histopathology of liver and kidneys. Methods: For sub-chronic toxicity study a total of 30 mice were used, three groups (I–III) of mice (10 animals each) were used. Group I served as control and received a vehicle while groups II and III daily administered with 700 and 2100 mg/kg extract respectively orally by using oral gavage for 90 days. At the end of the experiment, the mice were sacrificed by using diethyl ether; blood was collected for assessing biochemical parameters and histopathological evaluations on liver and kidneys were performed. Results: Sub chronic treatment of extract for 90 days, at 700 and 2100 mg/kg body weight did not induce any sign of illness and /or death and had no adverse effect on biochemical parameters and blood parameters. Liver and kidney sections also revealed normal architecture, except some pyknotic nuclei and focal mononuclear leukocytic infiltrations observed in some of the liver and kidney tissues at higher dose (2100mg/kg). Conclusion: The results of this sub chronic toxicity study showed that M. gracilipes aqueous leaves extract is safe at daily doses of 700 mg/kg body weight, even when taken for longer period. At higher doses, however, the extract may induce mild hepatorenal toxicity. VL - 12 IS - 2 ER -
Department of Anatomy, Institute of health, Jimma University, Jimma, Ethiopia
Department of Anatomy, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia
Department of Pharmacology, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
Department of Pathology, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
Department of Traditional Medicine, Ethiopian Public Health Institute, Addis Ababa, Ethiopia
Department of Physiology, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
Department of Biochemistry, Institute of Health, Jimma University, Jimma, Ethiopia
